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Vitamin B12 (cobalamin) is one of the 13 vitamins essential to human health and life. It is crucial for the construction of DNA, production of red blood cells and aids in the maintenance of a healthy nervous system. B12 deficiency damages the brain, spinal cord, peripheral nerves, and nerves of the eye. A B12 deficiency can result in symptoms ranging from severe anemia requiring blood transfusions, fatigue, muscle soreness, memory loss, and behavioral changes to serious and permanent nerve damage. B12 is found naturally in animal foods or is received through supplements, and must follow a complex pathway of several steps for proper absorption. Monobind's assay measures total B12 in serum.
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AccuBind® ELISA
Vitamin B12 AccuBind ELISA Kits
Available Materials
Item
Description
Price
Qty
Order
Product Specs
Method:
Enzyme Immunoassay, Colormetric
Principle:
Delayed-Competitive Assay, Streptavidin-Coated Plate
Calibrators:
0, 100, 200, 400, 1000, 2000 pg/ml
AccuLite® CLIA
Vitamin B12 AccuLite CLIA Kits
Available Materials
Item
Description
Price
Qty
Order
Product Specs
Method:
Enzyme Immunoassay, Chemiluminescence
Principle:
Delayed-Competitive Assay, Streptavidin-Coated Plate
Calibrators:
0, 100, 200, 400, 1000, 2000 pg/ml
Advisories
November 6, 2014 Advisory: Monobind recommends diluting high protein patient samples prior to performing extraction step. See IFU for update and Sample Preparation Guide, both linked on ELISA and CLIA product details pages.
July 21, 2014 Advisory: Releasing & Stabilization Agent volume fills have been increased to provide labs more reagents based on customer requests. See News or updated IFU for new volumes.
May 23, 2013 Advisory: Monobind has improved assay "ease of use" by adding red dye to the Neutralization Buffer which allows users to better distinguish extraction steps. The solution will be neutral after the addition of the first extraction reagent and red in color after the the addition of the neutralizing buffer in the second step. The procedure is otherwise unchanged.
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