Better Performance
The advantage of CLIA is "increased sensitivity & dynamic range, which allows detection of lower analyte concentrations & earlier diagnosis of disease." (IVD Technology Oct 2004). Monobind uses Luminol in its tracer reagent to enhance signal strength and produce a long-lasting glow reaction that is superior to other substrates. This results in an assay up to 100,000x more sensitive than ELISA.

CLIA's dynamic range is improved beyond ELISA with better linearity and discrimination of signal. While ELISA cannot read higher than 3.0 O.D. thus requiring sample dilution, CLIA can read a dynamic range of 106 or 107 significantly increasing assay linearity and reducing overflow. Further, low background (high signal-to-noise ratio) enables a clearer interpretation of low analyte concentrations.

Long Shelf Life
CLIA substrates have a shelf-life of 3 years, far superior to fluorescence and RIA, and like all AccuBind® ELISA products have a 18-month life at time of manufacturer with Monobind guaranteeing customers 12 months freshness from date of shipment.

Quicker Results
Incubation times are reduced from ELISA methods typically by 30 minutes or more, adding valuable time to technician schedules and increasing their productivity.

Easy Adoption
Monobind CLIA follows the same procedures as ELISA, with the exception of no stop solution and shorter incubation times, thereby making it easy for current Monobind ELISA users to adopt CLIA in full or partial analyte range. [same procedures links to view assay types]