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Immunoassays

Project Check List
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  1. Identify base plasma type
    1. Source  (typically 5-5.3% total protein)
    2. Fresh Frozen (higher total protein, typically 5.5-6%)
    3. Recovered (higher total protein, typically 5.5-6% with less intact enzymes/ protein)
    4. Off the Clot (higher total protein, typically 7% due to anticoagulant content)
      1. Pooled (individual units approx 5ml each combined)
      2. Single Donor (individual units approx 275ml each)
    5. Gendered (the above may be sorted by gender as available)
  2. Choose processing technique, or if unsure, tell us the intended use or other product specs (per #3 below). Available filtration methods after plasma defibrination are
    1. Charcoal Stripped (removes small molecules and some proteins from serum)
      1. Single process
      2. Double process (removes a higher percentage of molecules/proteins)
    2. Delipidated (removes triglycerides and cholesterol from serum)
    3. Charcoal Stripped, Delipidated (removes small molecules and some proteins as well as triglycerides and cholesterol)
      1. Single charcoal + delipidized
      2. Double charcoal + delipidized
    4. Resin Stripped (removes thyroid hormones from serum)
    5. Resin Stripped, Delipidated (removes thyroid hormones as well as triglycerides and cholesterol from serum)
  3. Specify analyte content levels, if available (this may affect the choice of base material or filtration methods), chemistry profile may include
    1. Total protein count
    2. Specific analyte values (eg enzymes, calcium, ALP etc)
    3. pH, electrolytes and other analytes ranges (provide targets)
  4. If desired, request preservatives be added (Monobind does not typically include therefore manufactured serum is shipped frozen)
  5. If desired, request specific product packaging
    1. Typically serum is packed in 3.5 liter jugs to reduce freight and ensure temperature consistency during transport
      1. Other sizes available
        1. 1 liter
        2. 10 liter
        3. Custom
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